Oxidative Phosphorylation Pogil

Where is the electron transport chain located?

In the electron transport chain, where is the highest concentration of hydrogen ions located?

How do hydrogen ions reach the intermembrane space?

Why is energy required to move the hydrogen ions across the membrane into the intermembrane space?

What molecules carry the high potential energy electrons used to pump hydrogen ions across the inner mitochondrial membrane?

Where did the electron acceptor molecules (NADH and FADH2) come from?

When electrons are released from the electron acceptor molecules, what else is produced?

Is the release of an electron from an electron acceptor molecule oxidation or reduction?

What molecule is the final electron acceptor after the electron has moved through the electron transport chain?

What compound is formed as a final product of the electron transport chain?

Is any ATP produced in the electron transport chain?

Is any ATP used in the electron transport chain?

Describe the movement of hydrogen ions through the membrane during chemiosmosis.

Would free energy be required for the hydrogen ions to move from the intermembrane space to the mitochondrial matrix?

What is the name of the embedded protein that provides a channel for the hydrogen ions to pass through the membrane?

The flow of hydrogen ions through the protein channel provides free energy to do work. What process in chemiosmosis requires energy?

During oxidation, what molecule is being phosphorylated?

Under ideal conditions each NADH molecule will result in 3 ATP molecules, and each FADH2 molecule will result in 2 ATP molecules during oxidative phosphorylation. Calculate the total number of ATP molecules that might be produced in this phase of cellular respiration from one glucose molecule.

Considering all the stages of cellular respiration (glycolysis, link, Krebs cycle, and oxidative phosphorylation) how many ATP molecules are produced from one glucose molecule, assuming ideal circumstances?

Because of its role in aerobic respiration, oxygen is essential for most living things on Earth. Describe the role of molecular oxygen in aerobic respiration.

In which phases is glucose used or produced?

In which phase is oxygen used or produced?

In which phase is carbon dioxide used or produced?

In which phase is water used or produced?

In which phases are the 38 ATP used or produced?

Where in the cell is glucose used or produced?

Where in the cell is oxygen used or produced?

Where in the cell is carbon dioxide used or produced?

Where in the cell is H2O used or produced?

Where in the cell is the 38 ATP used or produced?

Substrate level phosphorylation is the term used for phosphorylation that removes a phosphate from one molecule and joins it to another molecule. Oxidative phosphorylation is the term used for the attachment of free inorganic phosphate to a molecule. Identify the phases of cellular respiration that use substrate level phosphorylation and that use oxidative phosphorylation.

Which side of the inner mitochondrial membrane would have a higher pH?

During glycolysis the enzyme hexokinase uses ATP to transfer a phosphate to glucose to form fructose-diphosphate. Suppose that a cell has only glucose available for energy and that the activity of hexokinase is suddenly stopped. Explain in detail what is most likely to occur in the cell.

Prokaryote cells must have energy for cellular processes just like eukaryote cells do. Yet, they have no mitochondria. Which phase(s) of cellular respiration would be unaffected by the lack of mitochondria in a cell?

The link reaction and Krebs cycle occur in the cytoplasm of prokaryotes in the same way that they occur in the mitochondria of eukaryotes. However, a concentration gradient across a membrane is a requirement of the electron transport chain. Propose an alternative site for that phase of cellular respiration in prokaryotic cells.