MCB3020 Exam 1

24 July 2022
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Gram-positive bacteria have a thinner layer of peptidoglycan than gram-negative bacteria.
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False
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The concept that human and animal diseases are caused by microorganisms is called the
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Germ theory
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Who is credited with developing and documenting the first vaccination procedure against smallpox?
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Edward Jenner
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Although penicillin inhibits peptidoglycan synthesis, bacterial cells will continue to grow normally in the presence of penicillin in a(n) __________ environment.
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isotonic
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Which of the following contribute to the environmental resistance of bacterial endospores?
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-Lower pH -Low water content of endospores -Spore coat -Presence of calcium dipicolinate
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External structures that are found in Archaea but not Bacteria are _________.
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Hami
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Chemotaxis is a process by which bacteria
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move toward an attractant or away from a repellent
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The Archaea generally lack which of the following that are normally found in gram-negative bacteria?
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-A complex peptidoglycan network -Outer membrane -lipopolysaccharide
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Transport of two different substances can be linked. If the transport is in the same direction it is called __________; if the transport is in opposite directions it is called __________.
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Symport, antiport
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Although developed over 100 years ago, Koch's postulates continue to be used successfully in all known human infectious diseases.
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False
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Which of the following is not part of the process by which an endospore transforms to become a vegetative cell?
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Induction
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Which of the following are part of the process by which an endospore transforms to become a vegetative cell?
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-Outgrowth -Activation -Germination
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_______ is a peptidoglycan-like molecule found in the cell walls of some archaea.
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pseudomurein
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During the lag phase of microbial growth, the cells are metabolically inactive.
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false
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The most widely accepted current model for membrane structures is called the __________ model.
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fluid mosaic
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Penicillin inhibits bacterial growth by preventing the cross-linking during peptidoglycan synthesis. Why does penicillin not inhibit growth of archaeal cells?
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Most archaeal cells have a cell wall, but it is not composed of peptidoglycan.
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Resolution improves when the wavelength of the illuminating light decreases.
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True
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Molecules or regions of molecules that readily interact with water are said to be __________, whereas molecules or regions of molecules that are insoluble in water or do not readily interact with water are said to be hydrophobic.
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Hydrophilic
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In the Gram-staining procedure, the primary stain is
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Crystal Violet
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It was possible to view viruses only after the invention of the electron microscope because they are too small to be seen with a light microscope.
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True
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Small internal cell structures are best visualized with a
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Transmission electron microscope
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Organisms that require high levels of sodium chloride in order to grow are called __________ organisms.
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Halophilic
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The cell membrane is a rigid structure that provides bacteria with their characteristic shapes.
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False
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The cytoplasmic membrane of bacteria
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-acts as a selectively permeable barrier, allowing some molecules to pass while preventing the movement of others. -retains the cytoplasm and its contents. -is the major site of ATP synthesis in aerobes.
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Bacteria have a region of the cytoplasm known as the __________, which is not bounded by a membrane but contains the chromosome.
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nucleoid
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Microdomains in eukaryotic plasma membranes can participate in
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-Cell movement -Cell division -single transduction
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The lipopolysaccharide (LPS) that is found in the outer membrane of gram-negative bacteria is also known as
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Endotoxin
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Endospores represent a challenge to the fields of industrial and medical microbiology because
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endospores are resistant to harsh environments, thus allowing survival of endospore-forming organisms under conditions in which non-endospore-forming cells would not survive, and endospore-forming organisms are often dangerous pathogens.
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The bacterium E. coli swims in a straight line, called a __________, for a few seconds; then it stops, tumbles, then swims away in a new direction.
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run
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The movement of molecules across a membrane from an area of lower concentration to an area of higher concentration with the expenditure of energy is called
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Active transport
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Who of the following developed a set of criteria that could be used to establish a causative link between a particular microorganism and a particular disease?
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Koch
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Which of the following is/are true of capsules?
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-They retain water and help prevent desiccation of the bacteria. -They help bacteria escape phagocytosis by host cells. -They prevent entry of many bacterial viruses.
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The term used to describe bacteria that are shaped like curved rods is
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Vibrio
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The movement of molecules across a membrane from an area of higher concentration to an area of lower concentration with the use of a carrier molecule embedded in the membrane is called
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Facilitated Diffusion
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Gram-positive bacteria have a structurally and chemically more complex cell wall than gram-negative bacteria.
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False
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In the Gram-staining procedure, the counterstain is
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Safranin
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Bacterial cells that are variable in shape are called
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pleomorphic
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Which of the following is considered a cardinal growth temperature?
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-The optimum temperature -The maximum temperature -The minimum temperature
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A culture in a closed vessel to which no additional medium is added and from which no waste products are removed is called a __________ culture.
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Batch
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Rigid bacteria with a helical cell shape are called
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Spirilla
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Which of the following is useful in distinguishing between prokaryotic and eukaryotic cells?
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The presence of membrane-delimited organelles within the cytoplasm
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Immersion oil increases the amount of light entering the objective lens.
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True
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An instrument that magnifies slight differences in the refractive index of cell structures is called a (n) __________ microscope.
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Phase-Contrast
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Endospores are resistant to most environmental stressors because they have _____ complexed with calcium ions which stabilize DNA.
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dipicolinic acid
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The extreme insolubility of ferric iron leaves little free iron available for transport into bacterial cells.
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True
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Acid-fast organisms such as Mycobacterium tuberculosis contain __________ constructed from mycolic acids in their cell walls.
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Lipids
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Which of the following is a characteristic of active transport?
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-Can move materials against a concentration gradient -Use of ATP or a proton gradient as a source of metabolic energy -Saturable uptake rate
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Which of the following structures is NOT found in an endospore?
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Capsule
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A strain of Neisseria gonorrhoeae has undergone a mutation and is no longer able to make pili. Predict the most likely outc
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The bacteria will become less virulent and will not be able to readily establish infection.
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All of the following statements about the periplasmic space are true except:
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It is found only in gram-positive bacteria.
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All eukaryotes have a membrane-delimited nucleus.
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True
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Ribosomes are not considered to be organelles because they are not bounded by a membrane.
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True
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The term used to describe bacteria that have a spherical shape is
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coccus.
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Organisms that grow near deep-sea volcanic vents are likely to be
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Thermophilic
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The concept that living organisms arise from nonliving material is called
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Spontaneous generation
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Flexible bacteria with a helical shape are called
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spirochetes.
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Small circular DNA molecules capable of replicating and containing genes that are useful but not necessary to the bacterium are called __________.
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Plasmids
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Lipids with polar and nonpolar ends are said to be
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amphipathic
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The Gram-staining procedure is an example of ________________________.
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differential staining
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Endospores are reproductive structures.
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False
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Scanning electron microscopy is most often used to reveal
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Surface structures.
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How do spheroplasts and protosplasts differ?
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Spheroplasts retain their outer membrane but protoplasts do not. Meaning that spheroplast are gram-negative and protoplasts are gram-positive
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You discover a new transport system used by a newly discovered bacterial species. The sugars that are transported using this system are phosphorylated as they enter the bacterial cell. You would describe this transport system as a(n) _____.
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Group translocation
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The instrument that produces a bright image of the specimen against a dark background is called a (n) __________ microscope.
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Dark-field
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The total number of viable microorganisms remains constant in stationary phase because
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either there is a balance between cell division and cell death or there is a cessation of cell division even though the cells may remain metabolically active.
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After the primary stain has been added but before the decolorizer has been used, gram-positive organisms are stained __________ and gram-negative organisms are stained __________.
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purple; purple
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The movement of molecules from an area of higher concentration to an area of lower concentration without the participation of specific carrier molecules is called
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Passive diffusion
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Organisms that have their optimum growth pH between 0.0 and 5.5 are called __________.
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Acidophiles
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If you forgot to apply the safranin counterstain while performing a Gram stain, which outcome would you expect?
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Gram-negative bacteria would be unstained
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Nutrients can be concentrated from dilute solutions by
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active transport and group translocation.
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Cells with a relatively complex morphology that have a true membrane-delimited nucleus are called
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eukaryotes.
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Media containing some ingredients of unknown chemical composition are called __________ media.
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Complex
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Which of the following protects bacteria from lysis in dilute solutions and helps to determine their cellular morphology or shape?
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Peptidoglycan
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Which bacterial structure may survive if temperatures applied during food preservation processes are too low?
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Endospores
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Agar is an excellent solidifying agent for microbiological media because
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it is not degraded by most microorganisms and solid agar remains solid until the temperature is raised to 90°C, and liquid agar remains liquid if the temperature is lowered to 45°C are correct.
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A growth medium that favors the growth of some microorganisms but inhibits the growth of other microorganisms is a __________ medium.
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Selective
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All of the following are characteristics of spread plates EXCEPT
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spread plates that readily grow anaerobic bacteria.
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All of the following are characteristics of spread plates
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-colonies that grow on the surface of the agar. -spread plates that are typically inoculated with a bent glass rod. -specimens that are typically diluted prior to inoculation of a spread plate
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Bacterial and archaeal ribosomes are known as _____ ribosomes, based on their sedimentation coefficient.
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70S
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Eukaryotic ribosomes are known as _____ ribosomes, based on their sedimentation coefficient.
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80S
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The plasma membrane of eukaryotes contains all of the following EXCEPT
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Peptidoglycan
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The plasma membrane of eukaryotes contains all of the following
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-sterols -sphingolipids. -phosphoglycerides.
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Immersion oil can be used to increase the resolution achieved with some microscope lenses because it increases the __________ between the specimen and the objective lens.
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Refractive indexProteins that are loosely associated with the cytoplasmic membrane are called __________ proteins.
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Proteins that are loosely associated with the cytoplasmic membrane are called __________ proteins.
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Peripheral
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__________ is the process through which endospores are formed within a vegetative cell.
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Sporulation
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Gram staining divides bacterial species into two groups based on differences in cell wall structure.
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True
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Pasteur
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Swan-neck flask experiment Disproved spontaneous generation Pasteurization and fermentation
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Hooke
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Coined the term "cell"
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Semmelweis
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hand washing prevents disease
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Snow
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epidemiology - the investigation of how diseases spread
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Ehrlich
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Developed the drug Salvarsan to treat syphilis
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Fleming
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discovered the first antibiotic, penicillin
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Griffith
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Discovered genetic transformation - The process by which bacteria can take up "naked" DNA from their environment
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Watson and Crick
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discovered the double helix structure of DNA
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Three domains of life
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Archaea, Bacteria, Eukarya
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Five-kingdom system
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Monera, Protista, Fungi, Plantae, and Animalia
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Monera
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Bacteria and Archaea (Prokaryotes)
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Viruses
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Acellular and non-living