Chapter 20 Mastering Biology

Which enzyme(s) will produce a DNA fragment that contains the entire vgp gene (shown in red) and has "sticky ends"? Select all that apply. HindIII EcoRI HaeIII HindIII

Which enzyme(s) would cut the human DNA shown in Part A on both sides of the vgp gene, but not inside the gene?

which enzyme(s) would cut the plasmid without disrupting the function of the ampR gene?

Which enzyme(s) would produce sticky ends when cutting both the human DNA and the plasmid?

Which one resitriction enzyme satisfies all three of the requirements listed above?

Part C - Transformation and selection of recombinant bacteria

Part D - Screening the plasmid library

What information can not be obtained from the sequence of a gene?

What is the polymerase chain reaction (PCR)?

True or false? Comparison of the sequences of the same gene across species can give some insight into the existence of a common ancestor with that gene.

True or false? The Taq enzyme is a type of DNA polymerase that allows researchers to separate the DNA strands during the annealing step of the PCR cycle without destroying the polymerase.

How many DNA molecules would there be after four rounds of PCR if the initial reaction mixture contained two molecules?

During which step in the PCR cycle are nucleotides used?

During which step in the PCR cycle do primers form bonds with a single-stranded template?

The unpaired nucleotides produced by the action of restriction enzymes are referred to as _____.

The sticky end of the DNA restriction fragment shown here will pair with a DNA restriction fragment with the sticky end _____.

What defect causes pituitary dwarfism?

What is genetic cloning?

True or false? The use of growth hormone isolated from cadavers was banned mainly because there were not enough cadavers to supply the hormone.

Which of the following statements best describes the function of reverse transcriptase?

Which of the following steps would be performed last when screening a cDNA library?

How can large quantities of protein be produced from a bacterial colony containing the gene of interest?

True or false? The term "sticky ends" refers to the overhanging ends on DNA that are generated by restriction enzymes, which can base pair with any DNA molecules that contain complementary sticky ends.

In order to insert a human gene into a plasmid, both must _____

What enzyme forms covalent bonds between restriction fragments?

This is an animation of gel electrophoresis. Which of these DNA molecules is the shortest?

In gel electrophoresis DNA molecules migrate from _____ to _____ ends of the gel.

This is a DNA fingerprint exhibiting samples from a victim, two suspects, and the crime scene. Which of these DNA fragments is common to both the victim and Suspect 1?

This is a DNA fingerprint exhibiting samples from a victim, two suspects, and the crime scene. Which of these DNA fragments is common to both the victim and Suspect 2?

Why is Suspect 1 considered more likely to have committed the crime than Suspect 2?