Chapter 12 Biology Homework

7 September 2022
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Which step in this process requires use of restriction enzymes?
Which step in this process requires use of restriction enzymes?
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Step B
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When DNA from two sources is combined into one single piece of DNA, it is known as
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recombinant DNA
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When plasmids are used to produce a desired protein,
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the desired gene is inserted into the plasmid, and the plasmid is returned to the bacterium by transformation.
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Part complete Frequently, genetic engineers use plasmids, which are _____.
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small circular pieces of DNA found in bacteria
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The unpaired nucleotides produced by the action of restriction enzymes are referred to as _____.
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sticky ends
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The sticky end of the DNA restriction fragment shown here will pair with a DNA restriction fragment with the sticky end _____.
The sticky end of the DNA restriction fragment shown here will pair with a DNA restriction fragment with the sticky end _____.
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-ACGT
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"Sticky ends" are
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DNA fragments with single-stranded ends.
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"Sticky ends" are very useful in genetic engineering because they __________.
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provide a site for complementary base pairing so that pieces of DNA can be linked together
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Can you correctly place the labels in this diagram that summarizes the process of creating recombinant DNA?
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a. restriction enzyme b. restriction site c. sticky end d. DNA ligase e. recombinant DNA
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An advantage of using reverse transcriptase to prepare a gene for cloning is that
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the resulting DNA strand will lack introns.
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Cloning human genes into the plasmids of bacteria has enabled scientists to __________.
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use bacteria as "factories" for protein products
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Which is a transgenic organism?
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a rat that has had rabbit hemoglobin genes added to its genome
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Transgenic organisms are only scientifically or commercially useful if __________.
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the inserted ("foreign") gene is expressed in the host organism
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What does the process of gene therapy involve?
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It adds a functioning version of the defective gene to the cells of an individual.
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What is the function of the polymerase chain reaction (PCR)?
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It can make a large amount of DNA from a tiny amount.
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During the process of electrophoresis, the ________ functions like a molecular sieve, separating the samples according to their size.
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agarose gel
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What is the purpose of gel electrophoresis?
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It allows for the separation of fragments of DNA.
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The variation that occurs in 13 predetermined sites scattered throughout the human genome can be used to identify individual people. This DNA profile is possible because __________.
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each individual is likely to have the same sequence in the same places but with a different number of repeats