Working With The Laboratory Mouse AALAS

25 July 2022
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63 test answers

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question
Which of the following falls within the range of the total blood volume of adult mice?
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1.2 ml
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What is a problem that is often encountered with mice housed on wire mesh flooring?
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Foot injuries
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Which of the factors below affect the routine sanitation schedule in animal facilities?
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All of the Above
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What best describes the oral gavage procedure?
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The administration of fluid into the stomach through a feeding needle that is inserted into the mouth and threaded down the esophagus.
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Federal policies and guidelines require humane treatment of laboratory animals:
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All of the Above
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Which statement best describes the in vivo method of monoclonal antibody production?
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It uses mice to grow hybridoma cells on their peritoneal lining.
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The Guide for the Care and Use of Laboratory Animals provides:
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The basic information needed to develop an institutional occupational health and safety program.
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What is the easiest way to induce gas anesthesia for a mouse?
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Use an anesthetic induction chamber
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What is the recommended method for the production of monoclonal antibodies?
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In Vitro Techniques
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Which of the following statements is/are TRUE regarding preparation of a mouse for surgery? Check all that apply.
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•The skin should be disinfected around the incision site. •The fur should be removed around the incision site. •You should firmly pinch the mouse's foot or toe to check for a pain reflex before beginning surgery.
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Which of the following statements about identifying mice is NOT true?
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Ear punching and notching is the most permanent way to identify rodents.
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Which of the following represents good practice in oral gavage of mice?
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•The needle tip should be inserted behind the incisors. •The head of the mouse should be in vertical alignment with the esophagus during the gavage.
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The goal of appropriate animal care is to:
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minimize pain and distress where possible.
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Who is responsible for the humane treatment of experimental animals?
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All of the Above
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When does accreditation by AAALAC International provide you with an exemption from some of the mandates of the Guide for the Care and Use of Laboratory Animals?
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Under no circumstances
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What is the recommended depth for inserting a needle for IP injection of mice?
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0.5 cm
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Which of the following statements is TRUE?
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Rodents are susceptible to subclinical infections after surgery.
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Which of the following is an injectable anesthetic agent?
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Pentobarbital
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Which statement is true about mice in the age range of 6-10 days?
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Mammaries become visible on the females around 9 days.
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Which of the following methods is considered acceptable for euthanizing mice?
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Injection with barbiturates
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Typically, subcutaneous injections in mice:
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All of the Above
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Which type of bedding is recommended for use in postoperative housing for mice?
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Paper bedding material
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What is the age range when mice are weaned?
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21-28 days
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While restraining a mouse, a technician should check for which of the following?
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All of the Above
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Which measure can correct respiratory depression in anesthetized mice?
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All of the Above
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Which of the following represents an appropriate technique for subcutaneous injections in mice?
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Lift some skin on the back of the mouse, and insert the needle into the base of the tent.
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How are Salmonella spp. typically transmitted from animals to humans?
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By the fecal-oral route
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When picking up a mouse from a filter top cage, putting the wire bar lid sideways on the cage is helpful to:
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Reposition your grasp as you take the mouse out of the cage as it will tend to hold on to the wire bar lid.
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What potential problem(s) should you be aware of when mice are undergoing surgical procedures?
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All of the Above
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Which of the following would provide longer-acting pain relief in mice?
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Carprofen
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What is the goal of an occupational health and safety program?
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To protect individuals who have frequent contact with animals in a research facility
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A straight needle is preferred for oral gavage in a 18 gram mouse because it prevents trauma to the esophagus.
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True
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Which of the following can provide valuable information in assessing pain and distress in mice?
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All of the Above
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What statement regarding adjunctive methods of euthanasia is TRUE?
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They are methods that are acceptable as a follow-up procedure for ensuring death.
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What is NOT a recommendation for postoperative care of mice?
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Withhold feed during at least 12 hours following surgery.
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Which of the following statements is TRUE about injectable anesthetics compared with gaseous anesthetics used with mice?
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Injectable agents cause a greater depression of respiration in rodents.
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Which of the following statements is TRUE about pain in rodents?
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It is good to assume that procedures that are painful for humans are also painful for animals.
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Who is responsible for the humane treatment of experimental animals?
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All of the Above
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Which of the following applies to doxapram?
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It combats respiratory depression
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Which is a characteristic of microchips used to identify mice?
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A microchip is injected subcutaneously with a hypodermic needle.
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Which of the factors below affect the routine sanitation schedule in animal facilities?
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All of the Above
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What is a safe bleed volume for a 20-gram mouse?
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0.1-0.2 ml
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Your research must comply with the Guide for the Care and Use of Laboratory Animals if:
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Your institution receives funds from the National Institutes of Health (NIH)
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Which of the following is true about mice in the age range of 1-5 days?
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They are born hairless
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Peri-procedural care includes:
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All of the Above
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Why should an IP injection be made on the mouse's right side?
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It avoids puncturing the cecum
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What best describes Bupivacaine?
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Local anesthetic agent
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Which statement is true about the anogenital distance in mice?
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the anogential distance is greater in the male than in the female
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To track for the presence of pain or distress in mice over time, the baseline should be based on:
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The signs observed on normal, untreated animals.
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All of the following are practical methods of identifying mice EXCEPT:
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Leg band
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When producing monoclonal antibodies in vivo, what parameter should be considered?
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All of the Above
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Which of the following statements about caging for mice reflect the information that is in the Guide for the Care and Use of Laboratory Animals?
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Minimum cage height is 5 inches
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What is generally a superior anesthesia choice for mice undergoing major surgical procedures?
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Inhalation anesthesia
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Which of the following statements about oral gavage in mice is NOT true?
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Anesthesia of the mouse can increase esophageal trauma caused by the gavage.
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Compliance with environmental standards in facilities housing mice is generally the responsibility of:
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The animal care staff
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Which of the following statements about lymphocytic choriomeningitis (LCM) is False?:
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It does not affect tumor lines
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Which statement is true about mice in the age range of 11 to 14 days?
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Teeth are beginning to erupt at day 11
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In the mouse, IP injection should proceed only after aspiration has shown that the needle is properly positioned.
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True
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Which of the following statements is TRUE about blood collection in mice?
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Sedation causes vasodilation which helps make the tail vein easier to find.
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What group determines if a euthanasia method is acceptable for use in a particular species?
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American Veterinary Medical Association
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Which of the following statements is correct about IP injections of mice?
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The IP injection site is on the animal's right side, close to the midline, in a caudal quadrant of the abdomen.
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What is the function of an IACUC?
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All of the Above
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Which of the following statements is/are true regarding illumination in rooms housing rodents?
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All of the Above