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25 July 2022
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genetics
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the study of inherited genes and their variation; how a genotype affects the phenotype
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genotype
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the code in the DNA that determines the trait; refers to the combination of alleles (nucleotide sequence information)
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phenotype
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the observable, expressed trait; what is observed when the allele (instructions) are expressed
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DNA
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copies (replicates) itself, holds information for the production of a functional organism (expression), and changes (mutation and variation)
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chromosome
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tightly packaged DNA, we have 23 pairs
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alleles
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variants in the DNA sequence of genes
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what does a nucleotide contain?
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a sugar, phosphate, and nitrogenous base
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5 prime end
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top end of the DNA strand
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3 prime end
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bottom end of the DNA strand
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What holds the 2 hydrogenous bases together?
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hydrogen bonds
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histones
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proteins that help to condense and package up DNA
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helicase
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unwinds the DNA
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primer
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stretch of RNA that provides an open 3 end for extension
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ligase
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seals up the gaps in the sugar phosphate backbone
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Okazi fragments
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short stretches of newly synthesized DNA
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DNA polymerase
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proof reads and incorporates new nucleotides into the strand (RNA primer)
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What is DNA transcribed into?
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RNA
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what is RNA transcribed into?
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a protein
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where does translation happen?
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in the cytoplasm of the cell
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where does transcription happen?
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in the nucleus
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transcription
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process that makes RNA copy of DNA gene that is complimentary to one strand of the DNA helix
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gene expression
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making a protein from a gene (genotype to phenotype)
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3 main levels of gene regulation
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genome, transcription, translation
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epigenetics
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the study of heritable changes in gene function/expression that are not due to a change in DNA sequence (different phenotypes, same genotypes)