Mastering A&P Chapter 23 Lab

24 July 2022
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Made of protein and are catalysts
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Enzymes are _______. are catalysts made of protein made of protein, are catalysts, and permanently changed by the substrate permanently changed by the substrate made of protein and are catalysts
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Substrate
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The substance that the enzyme acts on is called its _______. monomer subunit product substrate
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Both the salivary glands and the pancreas
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Amylase is secreted by _______. the salivary glands the pancrease the stomach the small intestine both the salivary glands and the pancreas
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Disaccharide
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Maltose is a _______. complex carbohydrate disaccharide type of starch monosaccharide
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Boiling denatured the enzyme, but freezing had no effect
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Which of the following describes the effect of boiling and freezing? Freezing denatured the enzyme, but boiling had no effect. Boiling and freezing both denatured amylase. Boiling denatured the enzyme, but freezing had no effect. Boiling and freezing had no effect on amylase.
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Substrate is present
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What does a strong IKI result indicate? Substrate is present. Amylase activity is optimal. Product of the reaction is present. Amylase is working.
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Is detected using Benedict's reagent
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he presence of reducing sugars _______. is detected using Benedict's reagent is detected using IKI means that amylase has been denatured means that the substrate is present
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Tubes 7 and 8
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Which tubes demonstrated amylase activity with a small amount of reducing sugar produced? tubes 7 and 8 tubes 3 and 4 tubes 1 and 2 tubes 5 and 6
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Reddish brown
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When using the Benedict's assay, which color would indicate the most reducing sugar present? orange green blue reddish brown
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Starch and cellulose
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IKI is used to test for the presence of _______. cellulose protein starch and protein starch starch and cellulose
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Hydrogen and ionic bonds
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Substrates are held in the active site by _______. hydrogen bonds hydrogen and ionic bonds ionic bonds hydrogen and covalent bonds covalent bonds
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They are linked differently
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What is the difference between cellulose and starch? Cellulose is a polysaccharide, but starch is not. Starch is a polysaccharide, but cellulose is not. They are linked differently. They are in different locations in the animal cell.
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Enzyme/substrate specificity
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The purpose of this activity was to look at the effect of _______. enzyme/substrate specificity salt concentration on enzyme activity temperature on enzyme activity pH on enzyme activity
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All are matched correctly
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Which of the following enzyme/substrate combinations is matched INCORRECTLY? cellulose - cellulase starch - amylase peptides - peptidase All are matched correctly.
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Bacteria
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The cellulase in this experiment came from _______. fungi a plant an animal bacteria
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Amylase does not digest cellulose
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What did the results of tube 3 demonstrate? Cellulose is a substrate of amylase. Peptidase digests peptides. The amylase contained contaminating cellulase. Amylase does not digest cellulose.
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Amino acids
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The building blocks for peptides are _______. maltose nucleic and amino acids amino acids nucleic acids glucose
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Chief cells
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Pepsin is secreted by _______. acinar cells goblet cells salivary glands chief cells
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An enzyme and a peptidase
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Pepsin is classified as _______. an enzyme and a peptidase a peptidase a substrate an enzyme an enzyme and a substrate
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Changes from colorless to yellow
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When BAPNA is hydrolyzed, it _______. changes from colorless to yellow produces reducing sugar complexes with starch changes from yellow in color to colorless
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It was inactivated
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What occurred when pepsin was boiled? It was inactivated. The enzyme didn't work because the pH was too low. Its activity increased. The enzyme didn't work because the incubation temperature was too high.
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A peptidase, an enzyme that digests protein and a protein that digests protein
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Pepsin is _______. a peptidase an enzyme that digests protein a peptidase, an enzyme that digests protein and a protein that digests protein a protein that digests protein a peptidase and an enzyme that digests protein
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Tubes 2, 5, and 6
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Which test tubes were used to determine the optimal pH for pepsin activity? tubes 4, 5, and 6 tubes 1, 2, and 3 tubes 2, 5, and 6 tubes 2, 3, and 4
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2
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The optimal pH for pepsin activity is _______. 7 9 The activity was the same at a pH of 2, 7 and 9. 2
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All of the above are true
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Triglycerides _______. are fats dissolve poorly in water are digested by lipase are lipids All of the above are true.
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Increases the surface area of the triglycerides
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Adding bile to triglycerides _______. causes them to clump digests triglycerides into monoglyceride and two fatty acids increases the surface area of the triglycerides chemically modifies the triglycerides
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The small intestine
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Which of following does NOT secrete a lipase? the salivary glands the small intestine the pancreas the stomach
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More basic
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Prior to lipase digestion of triglycerides, the solution would be _______. less alkaline more acidic more acidic and less alkaline more basic
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Tubes 1, 5, and 6
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Which test tubes were used to determine the optimal pH for lipase activity? tubes 2, 5, and 6 tubes 1, 2, and 3 tubes 1, 5, and 6 tubes 2, 3, and 4
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Tube 1
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Which test tube had the highest lipase activity? tube 2 tube 4 tube 3 tube 1
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The pH of the stomach is too low and no bile is present in the stomach
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Why is gastric lipase NOT as active as pancreatic lipase? The pH of the stomach is too high. The pH of the stomach is too low. No bile is present in the stomach. The pH of the stomach is too high and no bile is present in the stomach. The pH of the stomach is too low and no bile is present in the stomach.
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Triglyceride
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The substrate of lipase is _______. triglyceride fatty acids monoglyceride bile