Bio 203 Exam 4 Questions

5 September 2022
4.7 (114 reviews)
63 test answers

Unlock all answers in this set

Unlock answers (59)
question
The figure above shows a simple metabolic pathway. According to Beadle and Tatum's hypothesis, how many genes are necessary for this pathway?
answer
2
question
What amino acid sequence will be generated, based on the following mRNA codon sequence?
answer
) met-ser-ser-leu-ser-leu
question
Which of the following is directly related to a single amino acid? the amino acetyl tRNA synthase
answer
the three-base sequence of mRNA
question
In the process of transcription....
answer
**DNA is replicated.
question
What does it mean when we say the genetic code is redundant?
answer
More than one codon can specify the addition of the amino acid.
question
Codons are three-base sequences that specify the addition of a single amino acid. How do eukaryotic codons and prokaryotic codons compare?
answer
Codons are nearly a universal language among all organisms.
question
Which of the following occurs in prokaryotes but not in eukaryotes?
answer
Concurrent transcription and translation.
question
The flow of information in a cell proceeds in what sequence?
answer
From DNA to RNA to Protein
question
A codon consists of _____ bases and specifies which _____ will be inserted into the polypeptide chain.
answer
- three, amino acids - Three nucleotide bases make up a codon and specify which amino acid comes next in the sequence.
question
Codons are part of the molecular structure of _____.
answer
MRNA
question
Transcription in eukaryotes requires which of the following in addition to RNA polymerase?
answer
Several transcription factors (TF's)
question
What is the function of RNA polymerase in transcription?
answer
RNA polymerase binds to DNA, separates the strands, then uses one of the strands as a template from which to assemble nucleotides into a complementary RNA strand.
question
Accuracy in the translation of mRNA into the primary structure of a polypeptide depends on specificity in the
answer
Bonding the anticodon to the codon.
question
Which of the following is the first event to take place in translation in eukaryotes?
answer
the small subunit of the ribosome recognizes and attaches to the 5' cap of mRNA
question
A signal peptide _____.
answer
Helps target a protein to the ER
question
tRNA Anticodon Amino Acid GGC Proline CGU Alanine UGC Threonine CCG Glycine ACG Cysteine CGG Alanine The anticodon loop of the first tRNA that will complement this mRNA is?
answer
3' GGC 5'
question
During elongation, which site in the ribosome represents the location where a codon is being read?
answer
(A) Site
question
Post-translational modifications of proteins may include the _____.
answer
Removal of introns???
question
What is the effect of a nonsense mutation in a gene?
answer
It introduces a premature stop codon into the mRNA.
question
Which of the following types of mutation, resulting in an error in the mRNA just after the AUG start of translation, is likely to have the most serious effect on the polypeptide product?
answer
A deletion of two nucleotides
question
Of the following, which is the most current description of a gene?
answer
A DNA sequence that is expressed to form a functional product: either RNA or polypeptide
question
Which of the following molecules are produced by transcription?
answer
Ribosomes and mRNA
question
Which of the following molecules is/are produced by translation? Include molecules that are subject to further modification after initial synthesis.
answer
RNA polymerase and Aminoacyl-tRNA synthetase
question
Which of the following is a protein produced by a regulatory gene?
answer
REPRESSOR
question
A mutation that inactivates a regulatory gene of a repressible operon in an E. coli cell would result in _____.
answer
continuous transcription of the structural gene controlled by that regulator
question
The trp repressor blocks transcription of the trp operon when the repressor _____.
answer
Binds to the tryptophan
question
CAP is said to be responsible for positive regulation of the lac operon because _____.
answer
CAP bound to the CAP-binding site increases the frequency of transcription initiation
question
The lac operon is most likely to be transcribed when
answer
When cyclic amp and lactose levels are high within the cell.
question
Imagine that you've isolated a yeast mutant that contains histones resistant to acetylation. What phenotype do you predict for this mutant?
answer
the mutant will show low levels of gene expression
question
The reason for differences in the sets of proteins expressed in a nerve and a pancreatic cell of the same individual is that nerve and pancreatic cells contain different _____.
answer
Sets of regulatory proteins
question
DNA methylation and histone acetylation are examples of _____.
answer
Genetic mutation?***
question
Which of the following is most likely to have a small protein called ubiquitin attached to it?
answer
a cyclin protein, that usually acts in G1, in a cell that is in G2
question
DNA methylation is a mechanism used by eukaryotes to do what?
answer
Inactivate genes
question
The phenomenon in which RNA molecules in a cell are destroyed if they have a sequence complementary to an introduced double-stranded RNA is called _____.
answer
RNA Interference
question
Among the newly discovered small noncoding RNAs, one type reestablishes methylation patterns during gamete formation and blocks expression of some transposons. These are known as _____.
answer
piRNA
question
The fact that plants can be cloned from somatic cells demonstrates that _____.
answer
Differentiated cells retain all genes of the zygote
question
If a Drosophila female has a homozygous mutation for a maternal effect gene, _____.
answer
All of her offspring will show mutant phenotype regardless of their genotype.
question
Which statement(s) about inducible operons is/are correct?
answer
-In an inducible operon, the repressor is synthesized in an active form -in an inducible operon an inducer inactivates the repressor.
question
Which of the following is a protein produced by a regulatory gene?
answer
Repressor
question
For a repressible operon to be transcribed, which of the following must occur?
answer
RNA polymerase must bind to the promoter and the repressor must be inactive
question
There is a mutation in the repressor that results in a molecule known as a super-repressor because it represses the lac operon permanently. Which of these would characterize such a mutant?
answer
it cannot bind to the inducer.
question
Suppose an experimenter becomes proficient with a technique that allows her to move DNA sequences within a prokaryotic genome. If she moves the operator to the far end of the operon, past the transacetylase (lacA) gene, which of the following would likely occur when the cell is exposed to lactose?
answer
the structural genes will be transcribed continuously.
question
Which of the following supports the argument that viruses are nonliving?
answer
They are not cellular
question
A lack of which molecule would result in a cell's inability to "turn off" genes?
answer
Coreporessor
question
What is the main structural difference between enveloped and nonenveloped viruses?
answer
Enveloped viruses have a phospholipid membrane
question
The nucleic acid of a virus particle is enclosed in a protein coat. What is it called?
answer
Capsid
question
The host range of a virus is determined by _____.
answer
The proteins on its surface and that of the host
question
In many ways, the regulation of the genes of a particular group of viruses will be similar to the regulation of the host genes. Therefore, which of the following would you expect of the genes of a bacteriophage?
answer
Control of more than one gene in the operon
question
Which of the following statements describes the lysogenic cycle of lambda (Ξ») phage?
answer
The phage genome replicated along with the host genome
question
Which viruses have single-stranded RNA that acts as a template for DNA synthesis?
answer
Retroviruses
question
Some viruses can be crystallized and their structures analyzed. One such virus is yellow mottle virus, which infects beans. This virus has a single-stranded RNA genome containing about 6300 nucleotides. Its capsid is 25-30 nm in diameter and contains 180 identical capsomeres. If the yellow mottle virus begins its infection of a cell by using its genome as mRNA, which of the following would you expect to be able to measure?
answer
Translation rate
question
The virus genome and viral proteins are assembled into virions (virus particles) during _____.
answer
?????? lysogenic cycle
question
HIV is inactivated in the laboratory after a few minutes of sitting at room temperature, but the flu virus is still active after sitting for several hours. What are the practical consequences of these findings?
answer
HIV can be transmitted more easily from person to person that the flu virus.
question
Which of the following human diseases is caused by a virus that requires reverse transcriptase to transcribe its genome inside the host cell?
answer
AIDS Virus
question
Why are retroviruses considered a special class of viruses?
answer
They transcribe RNA to DNA using reverse transcriptase
question
A researcher lyses a cell that contains nucleic acid molecules and capsomeres of tobacco mosaic virus (TMV). The cell contents are left in a covered test tube overnight. The next day this mixture is sprayed on tobacco plants. We expect that the plants would _____.
answer
Develop the typical symptoms of TMV infection
question
Which of the following can be effective in preventing the onset of viral infection in humans?
answer
Getting vaccinated
question
How does a virus differ from a bacterium?
answer
Viruses unlike bacteria lack metabolic enzymes
question
Why is it ineffective to treat viral disease with antibiotics?
answer
Antihiotics inhibit enzymes specific to bacteria and have no effect on virally encoded enzymes
question
What is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into a bacterium? I. Transform bacteria with a recombinant DNA molecule. II. Cut the plasmid DNA using restriction enzymes (endonucleases). III. Extract plasmid DNA from bacterial cells. IV. Hydrogen-bond the plasmid DNA to nonplasmid DNA fragments. V. Use ligase to seal plasmid DNA to nonplasmid DNA.
answer
III, II, IV, V, I
question
To introduce a particular piece of DNA into an animal cell, such as that of a mouse, you would most likely be successful with which of the following methods?
answer
Electroporation followed by recombination
question
Pax-6 is a gene that is involved in eye formation in many invertebrates, such as Drosophila. Pax-6 is also found in vertebrates. APax-6 gene from a mouse can be expressed in a fly and the protein (PAX-6) leads to a compound fly eye. This information suggests which of the following?
answer
Pax 6 is highly conserved and shows shared evolutionary ancestry.
question
A laboratory might use dideoxyribonucleotides to _____.
answer
Sequence a DNA fragment