Micro-Chapter 3

25 July 2022
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Light microscopy is
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the use of any kind of microscope that uses visible light to observe specimens
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Total magnification is
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the objective lens x ocular lens
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What is the function of the diaphragm on a compound microscope?
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controls the amount of light entering the condenser
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What is resolution?
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the ability of the lenses to distinguish two points
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The ocular lens is used to?
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magnify the image from the objective lens
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Brightfield illumination is produced by the
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condenser
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Which microscope would you choose to view the cellular contents of of a microbe in its natural state?
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phase-contrast microscope
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Which microscope uses a UV light source?
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fluorescence microscope
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Which microscope achieves the highest magnification and the greatest resolution?
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electron microscope
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Which microscope is especially useful in studying the surface structures of intact cells and viruses?
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scanning electron microscope
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Objects smaller than about 0.2 micrometers, such as viruses, must be examined with what type of microscope?
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electron microscope
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What is staining?
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coloring the microbe with a dye that emphasizes certain structures
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What is a smear?
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a thin film of a solution of microbes on a slide.
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Why are smear preparations fixed?
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to kill the microorganisms and fix them to the slide