LS23L Pre/In-Lab/Post Lab Quizzes

Which of these is not a variable that is mentioned as one that can be tested in the Metabolism lab? a. Nicotine b. Light/darkness c. pH d. Salinity e. Caffeine

There are many alternative ways to analyze and present the data gathered from this experiment (Metabolism). True False

One of the objectives of the Metabolism lab is to formulate a hypothesis that is: a. statistically significant b. using mammals c. testable d. complex

According to the lab safety sheet, the water used for the fish is treated with all of the following chemicals except except: a. Alphatic amine salts b. Sodium bicarbonate c. All of these chemicals are used d. EDTA e. Sodium thiosulfate pentahydrate

Because the rate of gas consumption will be measured, taking into account the mass of the organism will not have an effect on the interpretation of the data. True False

According to the lab safety sheet, if you get treated "fish water" in your eyes you don't have to rinse them because the water used for fish is non-toxic. True False

The fish metabolism experiment will be performed by placing fish in the test chamber and observe change in gas concentration through time. True False

Since we will be measuring the rate of metabolism, it will be unnecessary to monitor the behavior of the organism. True False

When working with the goldfish you must wear: (check all that apply) a. Standard lab attire b. Lab coat c. Respirator d. Gloves e. Goggles

Students are going to design an experiment flowchart with detailed steps of experimental procedures. True False

According to the Research Information Sheet, you are required to participate in the Memory Interference Test (MIT) in order to satisfactorily complete this course. True False

According to the Research Information Sheet, what is a benefit to subjects and/or society from participating in this study? a. You will help to develop better ways to evaluate cognition in people. b. There is no potential benefit from this experiment; it is purely a learning exercise. c. You will be guaranteed an A in the course and be able to become a scientist who will cure cancer. d. You will make your TA happy.

Choose the false statement concerning information from the Research Information Sheet. a. The purpose of this study is to build a database with which to practice experimental design. b. Your specific scores on the test will not be accessible to you or anyone else. c. You may opt out of answering any question on the pre-test questionnaire. d. The database will provide you with demographic information about all subgroups as long as they contain at least 2 people e. All demographic information and performance results will be kept strictly confidential.

How should you perform the Memory Interference Test (MIT)? Select one: a. Using two fingers only b. With your TA's help c. As if you are typing normally d. With your classmates' help e. Using your index finger only

According to the Research Information Sheet, possible risks you assume while participating in the Memory Interference Test (MIT) include: a. None of these b. Fatigue/tiredness c. All of these d. Broken bones e. Head injuries

What is the statistical test that will be used in this week's lab? a. Kruskal-Wallis test b. Chi-square test c. Rank-sum test d. Student's t-test e. No statistical test will be run on this data

According to the Research Information Sheet, you will be paid for participating in this study. True False

You should take as much time as possible to ensure that you answer each question on the MIT correctly. True False

Spectrophotometry provides a ______ measure of the concentration of DNA. a. qualitative b. quantitative

In the Polyacrylamide and Agarose Gels lab, when working with your protein or DNA sample you must wear: Select one: a. Standard lab attire b. Gloves c. Lab coat d. Safety goggles e. All of these

In this lab, the purpose of spectrophotometry is to measure protein activity. True False

What are the functions of SDS in gel electrophoresis for estimating protein sizes? I. Disrupts hydrogen bonding in proteins, linearizing the protein II. Provides an overall negative charge on proteins, making the migration distance on gel a function of only protein size III. Intercalates between the amino acids of the protein allowing it to be visualized on the gel a. III only b. I only c. I and II d. II only e. I, II, and III

According to the lab safety sheet, the agarose gels used in this lab are cast with GelRed, a less toxic alternative to ethidium bromide. True False

The combination of gel filtration and SDS-PAGE can reveal the ______ structure of a protein. a. primary b. quaternary c. tertiary d. secondary

Which method will you use in this lab to separate proteins based on size? a. Gel filtration b. SDS-PAGE electrophoresis c. RASMOL d. None of these e. Agarose gel electrophoresis

What is the optimum wavelength of UV light absorbed by protein? a. 280 b. 260 c. 680 d. 570 e. 320

According to the lab safety sheet, in the Polyacrylamide and Agarose Gels lab, used microcentrifuge tubes and pipette tips should be disposed of in the regular trash. True False

Which of these substances is usually used to visualize proteins on a polyacrylamide gel? a. Phenol red b. Aniline blue c. Alcian blue d. Coomassie blue e. Ethidium bromide

The ventral aspect is always toward the belly. True False

Which structure serves as the boundary between the thoracic cavity and the abdomen? a. Spleen b. Ribcage c. Vibrissae d. Diaphragm e. Medulla

In which structure does maturation and storage of sperm occur? a. Testis b. Prostate c. Vas deferens d. Seminal vesicles e. Epididymis

The anterior aspect is always toward the head. True False

Which of these structures are associated with the excretory (urinary) system? I. Uterus II. Ureter III. Urethra a. II only b. II and III c. I, II, and III d. I only e. III only

Only animal parts can be placed in the black bag at the front of the room - absolutely no pins, applicator sticks or dissecting tools. True False

Which piece of PPE is optional during the Rat Dissection lab: a. Standard lab attire b. Safety goggles c. Lab coat d. Gloves e. All of the above are mandatory

Because the rats we use are screened for infectious agents we can dispose of them in the regular trash. True False

What is the function of the spleen? a. Cellulose fermentation b. Embryonic development c. Removal of blood cells and immunity d. Bile production e. Energy storage

In which part of the digestive tract does most nutrient absorption occur? a. Duodenum b. Cecum c. Esophagus d. Jejunum/ileum e. Stomach

ddNTPs are labeled with fluorescent dyes in order to differentiate between the different nucleotides. True False

In DNA sequencing, how many different fluorescent dyes are used to label ddNTPs? a. 4 b. 1 c. 3 d. None e. 2

How long ago did our human haplogroups diverge? a. A million years ago b. Decades ago c. Tens of thousands of years ago d. A thousand years ago e. Hundreds of years ago

What is the ratio of ddNTP to dNTP in the nucleotide mixture for the sequencing reaction? Select one: a. 1 ddNTP to 10 dNTP b. 1 ddNTP to 50 dNTP c. 1 dNTP to 50 ddNTP d. All dNTP e. All ddNTP

Which of the following statements is NOT true of both PCR & DNA sequencing? a. Both require a primer for initiation b. Both are based on in vitro DNA synthesis c. Both use ddNTPs to prematurely terminate DNA synthesis d. Both use DNA polymerase to catalyze the reaction

Which type of gel is used in DNA sequencing? a. some other kind of gel b. polyacrylamide c. no gel is used - only computer analysis d. agarose e. both polyacrylamide and agarose

In a sequencing reaction, deoxyribonucleotides terminate a replicating segment of DNA, while dideoxyribonucleotides allow it to continue. True False

What is the name of the the output image of the sequencing process? a. electropherogram b. electroradiogram c. none of these d. electrochemogram e. electrospectrogram

Who is the scientist for whom the dideoxy sequencing method is named? a. Darwin b. Pfluegl c. Crick d. Sanger e. Watson

Which of the following is not a type of MIT that may be taken? a. Word b. Picture c. Audio d. Physical e. All of these are types of MIT that may be taken

Which of the following pieces of information does not need to be included when you label your plate for the epidemiology exercise? a. Continent b. Date c. Name d. Your age e. Section number

According to the lab safety sheet, because the bacteria used in the epidemiology activity is considered a BSL Risk Group 1 agent it is safe to throw any contaminated materials in the regular trash. True False

According to the lab safety sheet, the bacteria used in the epidemiology activity is considered a BSL Risk Group 1 agent and is not associated with disease in healthy adult humans. True False

Which of the following factors is mentioned as contributing to the spread of a disease? a. All of these are factors b. Natural environment of a region c. Genetic background of a population d. Infrastructure present in a nation e. Cultural and social practices

According to the lab safety sheet, in the Epidemiology & Laboratory Techniques lab all microcentrifuge tubes, pipette tips and cuvettes used during the pipetting exercises and at the practice stations can be disposed of in the regular trash. True False

How many rounds of handshaking will the epidemiology exercise have? a. 2 b. 3 c. 4 d. 1 e. 5

According to the lab safety sheet, during the epidemiology lab activity you must wear: a. Standard lab attire b. Safety goggles c. Lab coat d. Glove on you non-dominant hand e. All of the above

When setting the volume on a pipetter, you should first dial above the volume you want to pipet, then dial down to the correct reading. True False

The maximum volume for a pipetter can be found on the pushbutton. True False

1000 pg (picograms) is equal to how many ng (nanograms)? a. 0.1 b. 1 c. 10 d. 100 e. 1000

According to the Lab Safety Sheet for the b-galactosidase assay lab, it is okay to use chloroform at your bench work station as long as you always use the dropper bottle and wear gloves. True False

What is the purpose of Z buffer in the b-galactosidase lab? a. partially disrupts cell membrane to allow substrate to enter the cell b. provides an optimum pH for enzymatic reaction c. drastically changes the pH to stop the enzymatic reaction d. separates the bonding between enzyme subunits e. provides nutrient for bacteria growth

What is the purpose of sodium carbonate in the b-galactosidase lab? a. drastically changes the pH to stop the enzymatic reaction b. separates the bonding between enzyme subunits c. partially disrupts cell membrane to allow substrate to enter the cell d. provides an optimum pH for enzymatic reaction e. provides nutrient for bacteria growth

According to the Lab Safety Sheet provided for this week, when working with E. coli cells you must wear: (check all that apply) a. Safety goggles b. Gloves c. Standard lab attire d. Lab coat e. Full face air purifying respirator

What is the natural substrate of b-galactosidase? a. glucose b. ONPG c. galactose d. IPTG e. lactose

According to the Lab Safety Sheet provided for this week, which of the following is a potential hazard you will face in the b-galactosidase assay lab? (Check all that apply) a. Chemicals causing respiratory tract, eye and skin irritation b. Potentially carcinogenic chemicals c. Radioactive materials d. Water reactive chemicals e. Corrosive chemicals

What is the purpose of Luria broth in the b-galactosidase lab? a. drastically changes the pH to stop the enzymatic reaction b. separates the bonding between enzyme subunits c. provides nutrient for bacteria growth d. partially disrupts cell membrane to allow substrate to enter the cell e. provides an optimum pH for enzymatic reaction

What type of blank should be used for cell density assay? a. ONPG blank b. LB blank provided c. water blank d. Z buffer blank

What is the purpose of chloroform in the b-galactosidase lab? a. drastically changes the pH to stop the enzymatic reaction b. provides an optimum pH for enzymatic reaction c. provides nutrient for bacteria growth d. separates the bonding between enzyme subunits e. partially disrupts cell membrane to allow substrate to enter the cell

What is the purpose of ONPG in the b-galactosidase lab? a. substrate that gives a yellow color when cleaved b. inducer for the lac repressor c. provides nutrient for bacteria growth d. provides an optimum pH for enzymatic reaction e. separates the bonding between enzyme subunits

The instrument that you will use in the metabolism lab needs to be warmed up before use. True False

According to the lab safety sheet, the water used for the fish is treated with all of the following chemicals EXCEPT: a. Sodium thiosulfate pentahydrate b. Sodium bicarbonate c. EDTA d. Alphatic amine salts e. All of these chemicals are used

The Metabolism lab has a computer guide to help students develop a hypothesis. True False

Students are going to use a mammal model to test a hypothesis on metabolism. True False

One of the objectives of the Metabolism lab is to formulate a hypothesis that is: a. statistically significant b. testable c. complex d. using mammals

Because the rate of gas consumption will be measured, taking into account the mass of the organism will not have an effect on the interpretation of the data. True False

In the Metabolism in Goldfish lab, you must wear gloves to handle the fish. True False

The fish metabolism experiment will be performed by placing fish in the test chamber and observe change in gas concentration through time. True False

When working with the goldfish you must wear: (check all that apply) a. Lab coat b. Standard lab attire c. Respirator d. Goggles e. Gloves

What is the special property of Taq polymerase that allows it to work in PCR? a. It is bigger than other polymerases. b. It is cheaper than other polymerases. c. It is heat resistant. d. It is non-degrading. e. There is no special property.

Mitochondrial DNA is used to follow material inheritance and sequences on the Y chromosome is used to follow paternal inheritance. Why is it not practical to analyze paternal inheritance in this class? a. it is not impractical because Y chromosome sequences are present in both men and women. b. Because only men have a Y chromosome, the women in the class wouldn't be able to participate c. Because only men have a Y chromosome, the women in the class would contribute excess data. d. Because only men have a Y chromosome, the men in the class wouldn't be able to participate. e. None of these are valid reasons.

Once you have loaded your sample on the plate you can dispose of the tube containing your cheek cells by: a. Putting it in the regular trash. b. Placing it in a hazardous waste container. c. None of these are correct. d. Placing it in the freezer. e. Putting it in the autoclave bin.

The purpose of Chelex in this lab is to (select all that apply): a. Catalyze the addition of deoxynucleotides to the growing DNA strand b. Melt the DNA strands in preparation for amplification c. Prevent the DNA from being degraded d. Bind to the Mg++ in the DNA extract

Mitochondrial DNA can be inherited from either an individual's father OR mother. True False

According to the LS Core Lab Safety Sheet for this lab, which of the following is a potential hazard you will face in the DNA Isolation lab? a. Corrosive chemicals b. Flammable chemicals c. Carcinogenic (cancer causing) chemicals d. Chemicals causing eye irritation e. Water reactive chemicals

In the DNA Isolation lab, DNA will be collected from: a. Skin b. Cheek cells/saliva c. Urine d. Blood e. Bone marrow

The technique used for DNA amplification is: a. Polymerase chain reaction b. PRIMER 3 c. Gel electrophoresis d. BLAST

Which of these descriptions of PCR is not correct? a. In the first step of PCR, DNA parental strands are denatured at 94C. b. PCR requires a single primer c. PCR stands for polymerase chain reaction d. PCR is used to make more copies of DNA for sequencing e. Taq polymerase is used because it is resistant to heat denaturation

You can remove your lab coat once you are done with the wet lab portion of the DNA Isolation experiment, even if other people in the room are still working with Chelex. True False

What is the name of the method for separating charged molecules, such as proteins and nucleic acid, in an electrical field? a. Electroporation b. Electrophoresis c. Chemophoresis d. Electroseparation e. Denaturation

Which method will you use in this lab to separate proteins based on size? a. Gel filtration b. Agarose gel electrophoresis c. SDS-PAGE electrophoresis d. RASMOL e. None of these

Some of the chemicals/materials you will use in the Polyacrylamide and Agarose Gels lab include (check all that apply): a. Chloroform b. Ethidium bromide c. Tris glycine polyacrylamide gels d. None of these e. TGS (tris-glycine-SDS) buffer

The combination of gel filtration and SDS-PAGE can reveal the _______ structure of a protein. a. tertiary b. secondary c. primary d. quaternary

According to the lab safety sheet, which of the following is a potential hazard you will face in the Polyacrylamide and Agarose Gels lab? (Check all that apply) a. Corrosive chemicals b. Water reactive chemicals c. Chemicals causing respiratory tract, eye and skin irritation d. Carcinogenic chemicals e. None of these

According to the lab safety sheet, the agarose gels used in this lab are cast with GelRed, a less toxic alternative to ethidium bromide. True False

After the treatment of proteins described in the lab manual, all proteins used in this lab should carry an overall _____ charge. a. negative b. positive

The anterior aspect is always toward the head. True False

In order to cause the tail to move, which structures are pulled with the forceps? a. Tendons b. Ligaments c. Bones d. Nerves e. Muscles

In the rat, one lung has 2 lobes while the other has 3. True False

The medulla oblongata and the cerebellum are at the anterior of the brain. True False

All of the following are chemicals/materials present in the Rat Dissection lab except: a. Rats b. Scalpels c. Pins d. Chemical preservatives e. All of the above

In which structure of the female reproductive system does fertilization usually occur? a. Fallopian tubes b. Vagina c. Uterus d. Ovary e. Vas deferens

In order to inflate the lungs, which structure should be intubated (have tubing inserted through it)? a. Urethra b. Rectum c. Esophagus d. Trachea e. Ureter

By definition, histology is the study of: a. Microscopes b. Tissues c. Diseases d. Organs e. Allergies

Which of these is NOT a tissue type that you must match to a slide? a. Blood b. Bone c. Fat d. Neurons e. Lung

Larger specimens should be examined using the dissecting microscope. True False

Which of the following is not a tissue that you will examine using the dissecting microscopes? a. Brain b. Blood c. Muscle d. Liver e. Bone

The dissecting microscope has two lenses while the compound microscope has three. True False

When adjusting the compound microscope, you should always start from a low magnification and change to higher magnifications afterward. True False

The structure of organs and the tissues they are made up of is intimately related to their physiological function. True False

There are no chemicals used in the Histology lab. True False

Because you are just looking at slides and tissues this week you are not required to wear standard lab attire for the histology lab. True False

If a slide is broken you can throw the pieces in the regular trash as long as they are small. True False