Chapter 3 Microbiology

24 July 2022
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The concept of a prokaryote was first fully outlined in 1962 by
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Stanier and Van Niel
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The genus of bacteria that undergoes cell division in random planes, forming grapelike clusters of round bacteria, are the __________.
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staphylococci
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Bacteria that do not have a fixed shape are said to be __________.
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pleomorphic
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A branched network of hyphae formed by the Actinomycetes is called a __________.
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mycelium
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Actinomycetes form long filaments called ___________.
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hyphae
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The term used to describe bacteria that are intermediate in shape between spherical and rodshaped is
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coccobacilli.
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Bacterial cells that are variable in shape are called
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pleomorphic.
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Square planar arrangement of cells that forms when round bacteria remain attached to each other during reproduction are called
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tetrads.
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Flexible bacteria with a helical shape are called
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spirochetes.
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Rigid bacteria with a helical cell shape are called
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spirilla.
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The term used to describe bacteria that are shaped like curved rods is
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vibrio.
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The term used to describe bacteria that are rod shaped is
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bacillus.
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The term used to describe bacteria that have a spherical shape is
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coccus.
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Sterol-like molecules called hopanoids are thought to be important for the structural integrity of many bacteria because of their suspected role in membrane stabilization.
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TRUE
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The cytoplasmic membrane of bacteria
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A. retains the cytoplasm and its contents., B. acts as a selectively permeable barrier, allowing some molecules to pass while preventing the movement of others., C. is the major site of ATP synthesis in aerobes.
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The lipopolysaccharide (LPS) that is found in the outer membrane of gram-negative bacteria is also known as
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endotoxin.
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Which of the following is/are true of capsules?
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A. They help bacteria escape phagocytosis by host cells., B. They retain water and help prevent desiccation of the bacteria., C. They prevent entry of many bacterial viruses.
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Proteins that are loosely associated with the cytoplasmic membrane are called __________proteins.
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peripheral
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Proteins that are embedded within the cytoplasmic membrane and not easily extracted are called __________ proteins.
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integral
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Shrinkage of the plasma membrane away from the cell wall when the bacterium is placed in a hypertonic environment is called
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plasmolysis.
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Lipids with polar and nonpolar ends are said to be
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amphipathic.
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Although penicillin inhibits peptidoglycan synthesis, bacterial cells will continue to grow normally in the presence of penicillin in a(n) __________ environment.
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isotonic
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All of the following statements about the periplasmic space are true except: "It is located between the plasma membrane and outer membrane of gram-negative bacteria." "It contains hydrolytic enzymes and binding proteins involved in nutrient acquisition."
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it is found only in gram-positive bacteria.
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Which of the following protects bacteria from lysis in dilute solutions and helps to determine their cellular morphology or shape?
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peptidoglycan
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Sortase is a protein enzyme of bacteria that
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catalyzes covalent attachment of some surface proteins to peptidoglycan.
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The cell membrane is a rigid structure that provides bacteria with their characteristic shapes.
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FALSE
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Gram-positive bacteria have a thinner layer of peptidoglycan than gram-negative bacteria.
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FALSE
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Gram-positive bacteria have a structurally and chemically more complex cell wall than gramnegative bacteria.
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FALSE
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Bacteria growing at lower temperatures have more saturated fatty acids in their membranes.
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FALSE
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When the cell wall is removed from a Gram-negative bacterium without removing the outer membrane, the resulting form is called a(n) __________.
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spheroplast
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Enzymes that are secreted out of the cell to aid in the acquisition and digestion of environmental nutrients are called __________.
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exoenzymes
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Molecules or regions of molecules that readily interact with water are said to be __________, whereas molecules or regions of molecules that are insoluble in water or do not readily interact with water are said to be __________.
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hydrophilic, hydrophobic
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A __________ layer consists of diffuse unorganized polysaccharide material that lies outside the cell wall and is easily removed.
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slime
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A __________ is a polysaccharide layer that lies outside the cell wall and is not easily removed.
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capsule
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A(n) __________ layer is a layer of protein or glycoprotein that exhibits a pattern resembling floor tiles.
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S
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The most widely accepted current model for membrane structures is called the __________model.
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fluid mosaic
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The layers of peptidoglycan of the bacterial cell wall can be cross-linked by peptide intrabridges or by direct cross-linking.
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FALSE
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A feature unique to some archaeal plasma membranes is that they may
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consist of a lipid monolayer.
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Cell walls of most Archaea and bacteria contain peptidoglycan.
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FALSE
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_______ is a peptidoglycan-like molecule found in the cell walls of some archaea.
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Pseudomurein
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The hydrocarbons found in the membranes of archaea are identical to those found in bacteria.
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FALSE
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Which of the following is not true of bacterial plasmids?
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They are required for host growth and/or reproduction.
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Some photosynthetic bacteria produce gas vacuoles that regulate their buoyancy but contain no lipid.
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TRUE
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Intracellular granules of organic or inorganic material that are stockpiled by bacteria for future use are called __________.
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inclusions
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Bacteria have a region of the cytoplasm known as the __________, which is not bounded by a membrane but contains the chromosome.
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nucleoid
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Small circular DNA molecules capable of replicating and containing genes that are useful but not necessary to the bacterium are called __________.
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plasmids
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Some species of aquatic bacteria use inclusion bodies known as ________ to orient themselves in Earth's magnetic field.
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magnetosomes
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Bacterial and archaeal ribosomes are known as _____ ribosomes, based on their sedimentation coefficient.
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70S
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_______ are the site of protein synthesis in the cell.
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Ribosomes
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Sedimentation coefficients are proportional to the molecular weight of a particle and are not affected by the volume and shape of the particle.
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FALSE
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Bacterial flagella are composed of three parts: the ______ body, which is embedded in the cell, the hook, and the _______, which is the longest part of the flagellum.
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basal, filament
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The type III bacterial protein secretion machinery:
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A. has a needlelike structure through which proteins are secreted., B. includes structural features that may be evolutionarily related to the basal bodies of bacterial flagella., C. is found in gram-negative bacteria.
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Export of flagellin subunits is mediated by
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an apparatus in the basal body of the flagellum that is related to the type III secretion
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Proteinacious projections from the surface of a bacterium that are used to mediate conjugation are called sex __________, whereas projections that mediate attachment to surfaces such as host cells are called __________.
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pili, fimbriae
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During the assembly of the flagellar filament, the flagellin protein monomers assemble at the cell proximal base of the flagellum.
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FALSE
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Archaeal flagella are superficially similar to bacterial flagella, although archaeal flagella are much thicker than bacterial flagella.
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FALSE
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Matching: Match the definition with the word. 1) monotrichous, 2) peritrichous, 3) lophotrichous, 4) amphitrichous: a) a single flagellum, b) a single flagellum at each pole of an organism, c) a cluster of flagella at one or both ends of an organism, d) a relatively even distribution of flagella over the entire surface of the bacterium
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1-a, 2-d, 3-c, 4-b
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The power used by most flagellar motors is produced by
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proton motive force (PMF)
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Chemotaxis is a process by which bacteria
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move toward an attractant or away from a repellent.
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The rotation of bacterial flagella is powered by ATP hydrolysis.
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FALSE
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The bacterium E. coli swims in a straight line, called a __________, for a few seconds; then it stops, __________, then swims away in a new direction.
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run, tumbles
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In many spirochetes, multiple flagella combine to form a bundle known as a(n)____________ fibril, which winds around the cell and confers motility on the cell.
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axial
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Twitching or gliding motility involves
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both type IV pili and movement of slime can be involved in twitching or gliding motility.
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Endospores represent a challenge to the fields of industrial and medical microbiology because
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endospores are resistant to harsh environments, thus allowing survival of endo-sporeforming organisms under conditions in which non-endospore-forming cells would not survive, and endospore-forming organisms are often dangerous pathogens
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__________ is the process through which endospores are formed within a vegetative cell.
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Sporulation
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__________ is the main part of the process through which a vegetative cell is formed from an endospore.
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Germination
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The vegetative cell (mother cell) in which an endospore forms
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Sporangium
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The structural component of bacterial cell walls that is also called peptidoglycan
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Murein
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Plasma membrane and everything contained within
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Protoplast
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A unit of measure of the sedimentation velocity in a centrifuge
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Svedburg Unit
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A circular, double-stranded DNA molecule in prokaryotes that can exist and replicate independently of the chromosome
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Plasmid
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A gap between the plasma membrane and the cell wall of bacteria
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Periplasmic Space
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Organisms with peptidoglycan-like polymer with L-amino acids instead of D-amino acids
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Archaea
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Bursting of cells that occur when cells are placed in hypotonic solution so that water flows in the cell
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Lysis
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Well organized polysaccharides outside the cell wall that are not easily washed off
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Capsule
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Diffuse, unorganized polysaccharides outside the cell wall that are easily removed
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Slime Layer
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Protein or glycoprotein layer that exhibits a patter not unlike floor tiles
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S-Layer
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Hairlike appendages that are thinner than flagella and are usually involved in attachment rather than motility
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Fimbriae
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A flexible filament that is used for motility by spirochetes
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Axial Filament
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Movement toward chemical attractants or away from repellents
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Chemotaxis
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A structure formed by some species of bacteria that is resistant to some environmental stress
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Endospore
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The process of forming endospores within a vegetative cell
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Sporogenesis
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A network of polysaccharides extending from the surface of bacteria and other cells
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Glycocalyx
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Inclusion bodies that serve as a reserve of enzymes and site of CO2 fixation
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Carboxysomes
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Inclusion bodies that store extra nitrogen for bacteria
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Cyanophycin granules
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A polymer of glucose units; it serves as a carbon storage reservoir for many bacteria
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Glycogen
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A plasmid that can exist independent of the host chromosome or be integrated into it
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Episome
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A plasmid that has genes for pili and can transfer copies of itself to other bacteria
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Conjugative plasmid
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A plasmid that carries genes that encode resistance to antibiotics
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R factor
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A plasmid that carries genes for a toxin, and thus renders the host bacterium pathogenic
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Virulence plasmid
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A plasmid that carries genes for enzymes that degrade environmental substances such as aromatic compounds and pesticides
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Metabolic plasmid