BIO 101 (Unit 13)

25 July 2022
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question
What is the relationship between the template strand and the mRNA transcribed from it?
answer
The mRNA is complementary and runs in the reverse direction relative to 5' and 3'.
question
During translation, which component is directly responsible for reading the sequence of nucleotides so it may be translated into a sequence of amino acids?
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tRNA
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The picture below shows a ribosome translating mRNA. What event will happen next?
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The met amino acid will be attached to the val amino acid.
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In eukaryotic cells the first step in protein synthesis is the _____.
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transferring of information from DNA to messenger RNA
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The operon model of the regulation of gene expression in bacteria was proposed by _____.
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Jacob and Monod
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Which of these is NOT a component of the lac operon?
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regulatory gene only
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Regulatory proteins bind to _____.
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the operator
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In the presence of a regulatory protein the lac operon is _____.
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not transcribed
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Which of these is a regulatory gene?
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D
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In the diagram below, the gray unit represents _____.
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RNA polymerase
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In the diagram below, the green unit represents _____.
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the promoter
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In the diagram below, the two blue strands represent _____.
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DNA
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Which of these correctly illustrates the pairing of DNA and RNA nucleotides?
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GTTACG CAAUGC
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The direction of synthesis of an RNA transcript is _____.
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5' β€”> 3'
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Which of the following is LEAST likely to be a health problem for someone with cystic fibrosis?
answer
obesity
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The allele that causes cystic fibrosis is recessive. An individual who is heterozygous for cystic fibrosis
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is a carrier
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If Sophie conceives a child, and the father neither has cystic fibrosis nor is a carrier, what are the chances that the child would have cystic fibrosis?
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0%, because the child must receive the bad gene from both parents
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If Sophie has a brother or sister from the same parents, what are the chances that sibling would have cystic fibrosis?
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25%
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If someone is a carrier of a recessive genetic disorder, it means he or she has __________ of the allele, and __________ exhibit symptoms of the disorder.
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one copy; does not
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Which of the following do you think contributed the most to the success of Rhys' gene therapy?
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Doctors were replacing only one gene, and they only had to insert that gene into bone marrow cells.
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Theoretically, which of the following diseases should be the easiest to treat through gene therapy, considering the targeted organs and tissues where the corrected gene must be delivered?
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diabetes, since the target cells are found only in the pancreas
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Which of the following is most likely in a fetus lacking adequate amounts of the protein REST?
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Neurons will develop improperly.
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You are a neurologist. A patient presents to you with amyloid plaques. Which of the following is true?
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The patient may or may not exhibit symptoms of Alzheimer's.
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Which of the following people is most likely to have the highest levels of REST?
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A healthy 91 year old man.
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You are a neurologist doing research on Parkinson's disease. You discover something very similar to REST that impacts people with Parkinson's. What have you discovered?
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a protein
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Which of the following is true?
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REST is found in a wide variety of animals.
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Which of the following statements about ribosomes is correct?
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Ribosomes are made from RNA and protein. They are used to synthesize protein.
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What is another name for RNA synthesis?
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transcription
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The genetic code is often described as redundant. What feature of the genetic code does this reference?
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Most amino acids have more than one codon.
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If transcription and translation were applied to a book, which statement would correctly describe the process of each?
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Transcription is making a copy and translation is changing the language.
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Which of the following changes will have the least impact on a gene?
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an inversion mutation that inverts the gene and promoter
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Which of the following mutations will have the largest effect on the expression of this gene? 1 2 3 4 5 ATG GTC GCA TGT TAG.
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deleting the A in position 1
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Consider the DNA base sequences represented here. Which hypothetical substitution is most likely to be neutral? 1 2 3 4 5 ATG ACT TAT ACC TGA.
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substitution at the T labeled "2"
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What does it mean if a gene is "expressed"?
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It is transcribed and translated.
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The protein pepsin is produced in the cells lining the stomach, but not in cheek cells. Why is this?
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There are no transcription factors for pepsin in cheek cells.
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What would cause a region of DNA to have no expressed genes?
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if the DNA was condensed
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Eukaryotes use mRNA to carry the sequence for a protein to the ribosomes outside of the nucleus. Why then don't prokaryotes translate DNA directly?
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because control of transcription of mRNA is a major method of controlling gene expression
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Which of the following is a good reason for mRNA to be single-stranded, instead of double-stranded like DNA?
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because a complementary strand of RNA would prevent the binding of tRNA anticodons to the codon
question
You are a genetic engineer working on Zika. You develop a gene for causing populations of Aedes aegyptii to die. Which of the following did you create?
answer
crash drive
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Your cousin is developing a way to control Lyme disease, which is caused by bacteria and spread by ticks. With currently available technology, which of the following will he be altering with gene drives to achieve his goal?
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only the ticks.
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Which of the following is currently true?
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Gene drives would work on fast reproducing mice, but not on slow reproducing sloths.
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In order to make this process work, DNA must be altered where?
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germ line
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You study a genus of bacteria and discover an enzyme that behaves much like Cas9. What have you found?
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endonuclease
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You go to the grocery store. Which of the following genetically engineered foods will you be able to purchase there?
answer
salmon
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Which of the following is true?
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Farmers could use conventional breeding to increase the population of dairy cows that lack horns.
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You are a genetic engineer working on developing pigs that produce less waste. You want to use the most powerful gene editing technology available. What do you use?
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Crispr-Cas9
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Which of the following is true?
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DNA transfer works between members of the same species and members of different species.
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Which of the following is true?
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Genetic engineering was successfully done on plants before animals.
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If using these genetically modified mosquitoes is a success, which of the following will happen in Brazil?
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Cases of Zika and dengue fever will decrease.
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Which of the following natural methods would likely work the best to decrease the mosquito population around your home?
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Eliminate all standing water.
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You are a genetic engineer interested in using the same technique used in this method to control the Anopheles mosquito which transmits malaria. Which of the following do you release in malaria-riddled regions?
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engineered males
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Which of the following is the key to this project being a success?
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Following the release of the engineered mosquitoes, the offspring born in the wild will live, but will die before reproducing.
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If these mosquitoes are released in Florida and something goes wrong, which of the following agencies will be held responsible?
answer
Food and Drug Administration (FDA)
question
How did some stickleback populations come to live exclusively in fresh water?
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Some stickleback populations became trapped in lakes that formed at the end of the last ice age.
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Why do some stickleback populations lack pelvic spines?
answer
In lakes where there are no large predatory fish, there is no advantage to having pelvic spines. In lakes with dragonfly larvae, pelvic spines can be disadvantageous, allowing the predatory larvae to grab the fish.
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Why did Kingsley and his team cross marine and freshwater sticklebacks?
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To find the location of the gene(s) causing the difference between stickleback populations with and without spines.
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What did researchers discover about the genetic mutation causing the loss of pelvic spines?
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It is found in a regulatory region (a "switch") upstream of the coding region of the Pitx1 gene. It occurred in a similar DNA region in freshwater stickleback populations all over the world.
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Bell and collaborators painstakingly documented a population of fossil sticklebacks from an ancient freshwater lake over a 20,000-year period. The prevalence of sticklebacks with full and reduced pelvises changed over time. Which is true?
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The population of fish with pelvic spines that arrived in the lake at time B evolved a reduced pelvis over time (beginning at time C).
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How do multiple lines of evidence (from the field, the fossil record, and molecular genetics) work together to illustrate stickleback evolution?
answer
Data obtained by analyzing living fish in lakes show the selective pressures present in different environments. Genetic evidence reveals the precise molecular mechanism responsible for the change in pelvic structures in stickleback populations. If the same morphological changes occur in the fossil record as in living populations, we might deduce that the genetic mechanism discovered in the living populations might be responsible for the changes observed in fossils. The fossil data show a pattern of evolution over long stretches of time.
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What is the primary job of DNA?
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It provides blueprints for proteins.
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Bacteria simultaneously transcribe and translate mRNA. Why don't eukaryotes?
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Eukaryotic mRNA must be taken outside the nucleus before it can be translated.
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If the sequence for a eukaryotic gene is placed directly into a bacterial chromosome, what will occur?
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The bacterium will produce mRNA, but it will contain extra sequences not found in the eukaryotic mRNA due to transcription of both introns and exons by the bacterium.
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Which anticodon would match the codon AAG?
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UUC
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What is the function of the cap and tail on eukaryotic mRNA?
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They are involved in transporting the mRNA outside of the nucleus.
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What type of mutation is caused by adding one nucleotide into the middle of a sequence?
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insertion
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What prevents women from expressing genes on their two X chromosomes twice as much as men express their single X chromosome?
answer
Cells in women have one X condensed.